The Varian 500 MHz (VNMRS500) with two-channel NMR spectrometer equipped with 3-mm two-channel probe with gradient accessory was acquired in 2007. In March 2019, this instrument was upgraded with 1H CryoProbe CP217 (triple resonance 1H, 13C, or 15N).
The mode of operation for this instrument is similar to that of the 400 MHz instrument, except that CryoProbe is added to this spectrometer so that users can run experiments with reduced spectrometer acquisition time.
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Most commonly used experiments on VNMRS500 to characterize small molecules and smaller size biomolecules: Homonuclear (1H) and heteronuclear (1H, 13C, or 15N) 1D (1H, 13C-DEPT, APT, 15N) and 2D NMR experiments: COSY, DQF-COSY, NOESY, ROESY, TOCSY, 1H-13C HSQC, 1H-15N HSQC, 1H-13C HMBC, INADEQUATE, and diffusion ordered spectroscopy (DOSY).
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Sensitivity specifications: The probe sensitivity for 1H is 2235:1; 13C is 140:1; and 15N is 35:1.
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NMR software used: VNMR 4.2