PharmNMR Bruker 600 AV4

The state-of-the-art Bruker 600 AV4 NEO CryoProbe delivers unprecedented signal-to-noise (S/N) or sensitivity gain up to 4x and lower detection limits over a room-temperature probe. This signal intensity gain translates into up to ~ 20x reduction in data collection time for high-resolution liquid applications. The 5-mm 1H-optimized triple-channel CryoProbe is designed for 1H observation with 13C/15N decoupling and 13C observation with 1H decoupling due to superior sensitivity on 13C. This CryoProbe includes cryogenically cooled preamplifiers for 1H, 19F, 13C, 15N, and 2H. The increased sensitivity resulting from the cold preamplifier for 15N is especially important for the study of intrinsically disordered proteins, which some of our users propose to do. 19F nuclei can also be observed through the 1H channel for 19F-tagged proteins or small molecules with the highest possible sensitivity, which some of our users are also proposing to achieve. There are many Carbon detected experiments that can be done for those who are interested to investigate their intrinsically disordered proteins (IDP) structures and dynamics studies.

The Bruker 600 MHz NMR instrument is primarily for running experiments for biological research and for small molecules obtained in low micro-gram quantity. This state-of-the-art equipment is an extremely powerful device that will enable researchers to analyze increasingly complex biological systems such as the structure and conformational dynamics of biomolecules, protein folding, slow and fast kinetics of biomolecules, hydrogen-deuterium (H-D) exchange, and chemical exchange. The state-of-the-art- Bruker 600 MHz instrument is a powerful technique for determining the 3D structures of proteins, nucleic acids, and complex structures, as well as for monitoring and characterizing intermolecular interactions. The Bruker 600 MHz NMR instrument can be utilized to study protein-protein and protein-ligand interactions to better understand their biological functions in solution.

UK PharmNMR Instrument Sensitivity for Nuclei